Next-Gen DNA Sequencing
Also known as High-Output DNA Sequencing or Deep Sequencing. The MiSeq, NextSeq, and NovaSeq systems can generate highly accurate results in under a week for discoveries in genomics, epigenomics, gene expression analysis, and protein-nucleic acid interactions.
Speed & Simplicity for Targeted & Small Genomes
The MiSeq desktop sequencer allows you to access more focused applications such as targeted gene sequencing, metagenomics, small genome sequencing, targeted gene expression, amplicon sequencing, and HLA typing. New MiSeq reagents enable up to 15 Gb of output with 25 M sequencing reads and 2x300 bp read lengths.
Power & Efficiency for Large-Scale Genomics
The NovaSeq 6000 has been traded in for a NovaSeq X Plus. We will continue to run the 6000 until mid March 2024, when it will be returned. The X Plus provides equivalent or greater amounts of data at a lower cost. Please contact us at tucf-genomics@tufts.edu to discuss considerations for transitioning the 6000 to the X Plus.
Currently we offer either 100 or 150 nucleotide single or paired-end (50x2, 100x2, 150x2) reads. All sequence data, including reads mapped to a reference genome, will be available for download from our FTP site. Sequence data is archived on our server for one year. Additional bioinformatics support is available free to all Tufts users. We also offer bioinformatics consultations and support to outside institutions on a per-case basis.
Applications include:
- Whole Genome Sequencing
- De Novo Sequencing, Resequencing
- Deep Sequencing of Microbiomes
- Chromatin IP analysis (ChIP-Seq)
- Transcriptome Analysis (RNA-Seq)
- Small RNA Identification and Quantitation
- DNA Methylation analysis
- Metagenomics
All samples will be entered into a queue on receipt, and then scheduled for the next
available run. At current levels, your samples will be run within 2-3 weeks. Once your run
has started, turnaround time will be about a week.
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