TUCF Genomics introduces Next-Gen DNA Sequencing

The HiSeq instrument is being shutdown on 11/30/2022

Illumina has obsoleted the HiSeq and will stop selling HiSeq reagents on 11/30/2022, please reach out to us as we may have a few kits leftover for the HiSeq 2500. The NovaSeq is the replacement instrument for equivalent or greater amounts of data. Please contact us at tucf-genomics@tufts.edu to discuss considerations for transitioning from the HiSeq to the NovaSeq.

We are now offering NovaSeq 6000 services! Please inquire via email at tucf-genomics@tufts.edu Please also note that post covid have resumed our operation at full capacity with shifted schedule. Due to the schedule change, the timing of our email response may be slightly delayed. We also ask that if possible if you are dropping off samples that you schedule the drop off between 9AM and 2PM via email, if you would like to drop off outside this window please reach out to us at tucf-genomics@tufts.edu to arrange. The drop off location would be on the 8th floor of Stearns at 136 Harrison Ave. Please call us if you have any issues with Security and someone will meet you at the front of the building.

Also known as High-Output DNA Sequencing or Deep Sequencing. The MiSeq, NextSeq, and NovaSeq systems can generate highly accurate results in under a week for discoveries in genomics, epigenomics, gene expression analysis, and protein-nucleic acid interactions.

Speed & Simplicity for Targeted & Small Genomes

The MiSeq desktop sequencer allows you to access more focused applications such as targeted gene sequencing, metagenomics, small genome sequencing, targeted gene expression, amplicon sequencing, and HLA typing. New MiSeq reagents enable up to 15 Gb of output with 25 M sequencing reads and 2x300 bp read lengths.

Power & Efficiency for Large-Scale Genomics

The HiSeq instrument is being shutdown on 11/30/2022 Illumina has obsoleted the HiSeq and will stop selling HiSeq reagents on 11/30/2022 so at that time we will stop taking orders for HiSeq runs. The NovaSeq is the replacement instrument for equivalent or greater amounts of data. Please contact us at tucf-genomics@tufts.edu to discuss considerations for transitioning from the HiSeq to the NovaSeq or NextSeq.

Currently we offer either 50, 100 or 150 nucleotide single or paired-end (50x2, 100x2, 150x2) reads. All sequence data, including reads mapped to a reference genome, will be available for download from our FTP site. Sequence data is archived on our server for one year. Additional bioinformatics support is available free to all Tufts users. We also offer bioinformatics consultations and support to outside institutions on a per-case basis.

Applications include:

Whole Genome Sequencing
De Novo Sequencing, Resequencing
Deep Sequencing of Microbiomes
Chromatin IP analysis (ChIP-Seq)
Transcriptome Analysis (RNA-Seq)
Small RNA Identification and Quantitation
DNA Methylation analysis
Metagenomics

All samples will be entered into a queue on receipt, and then scheduled for the next available run. At current levels, your samples will be run within 2-3 weeks. Once your run has started, turnaround time will be about a week.