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TUCF Genomics introduces Next-Gen DNA Sequencing

Illumina_miseq

Also known as High-Output DNA Sequencing or Deep Sequencing. The MiSeq & HiSeq 2500 systems can generate highly accurate results in under a week for discoveries in genomics, epigenomics, gene expression analysis, and protein-nucleic acid interactions.

Speed & Simplicity for Targeted & Small Genomes

The MiSeq desktop sequencer allows you to access more focused applications such as targeted gene sequencing, metagenomics, small genome sequencing, targeted gene expression, amplicon sequencing, and HLA typing. New MiSeq reagents enable up to 15 Gb of output with 25 M sequencing reads and 2x300 bp read lengths.

Power & Efficiency for Large-Scale Genomics

The HiSeq 2500 System is a powerful and efficient ultra-high-throughput sequencing system that supports the broadest range of applications and study sizes. Unrivaled data quality using Illumina's proven SBS chemistry has made the HiSeq 2500 the instrument of choice for all major genome centers and leading institutions throughout the world.

Illumina_hiseq2500

Currently we offer either 50, 100 or 150 nucleotide single or paired-end (50x2, 100x2, 150x2) reads. All sequence data, including reads mapped to a reference genome, will be available for download from our FTP site. Sequence data is archived on our server for one year. Additional bioinformatics support is available free to all Tufts users. We also offer bioinformatics consultations and support to outside institutions on a per-case basis.

Applications include:

  • Whole Genome Sequencing
  • De Novo Sequencing, Resequencing
  • Deep Sequencing of Microbiomes
  • Chromatin IP analysis (ChIP-Seq)
  • Transcriptome Analysis (RNA-Seq)
  • Small RNA Identification and Quantitation
  • DNA Methylation analysis
  • Metagenomics

All samples will be entered into a queue on receipt, and then scheduled for the next available run. At current levels, your samples will be run within 2-3 weeks. Once your run has started, turnaround time will be about a week.