The sheer volume of data generated using high-throughput sequencing creates many
informatic challenges. We offer bioinformatics support associated with data management,
read mapping, individual consultations, and extended analysis. We also can point you in
the right direction with a variety of services.
|Data Analysis & Consultation
||TMC & External
|CLC Genomics Workbench
Analysis of genomics sample against reference genome generating SNP/DIP and alignment summary report.
|$50 per sample
||$75 per sample
|Custom Bioinformatics Projects
(i.e. Qiime, DeNovo Assembly, Whole Transcriptome Analysis, etc.)
Please contact us to discuss your project and for an estimate.
|$50 per hour
||$120 per hour
CLC Genomics Workbench Software
CLC Genomics Workbench performs both de novo assembly and super-fast reference assembly on hybrid data (454, Heliscope, Illumina GA,
SOLiD, Sanger) of any genome size. It has full support for bar coded data and full visualization of contigs with all NGS reads and
their quality scores.
CLC Genomics Workbench supports amongst others also
- Detection of single nucleotide and indel polymorphisms.
- Mapping of whole transcriptome and tag-based sequencing reads, identification of putative exons, and expression analysis.
- ChIP-seq experiment analysis.
For more info: CLC Genomics Workbench Software
CLC Sequence Viewer Software - (Free)
A Sequence Viewer for basic bioinformatics.
For more info: CLC Sequence Viewer Software
Bowtie is an ultrafast, memory-efficient short read aligner. It aligns short DNA sequences (reads) to the human genome at a rate of over
25 million 35-bp reads per hour. Bowtie indexes the genome with a Burrows-Wheeler index to keep its memory footprint small: typically
about 2.2 GB for the human genome (2.9 GB for paired-end).
For more info: http://bowtie-bio.sourceforge.net/
SAM (Sequence Alignment/Map) format is a generic format for storing large nucleotide sequence alignments. SAM aims to be a format that:
- Is flexible enough to store all the alignment information generated by various alignment programs
- Is simple enough to be easily generated by alignment programs or converted from existing alignment formats
- Is compact in file size
- Allows most of operations on the alignment to work on a stream without loading the whole alignment into memory
- Allows the file to be indexed by genomic position to efficiently retrieve all reads aligning to a locus
SAM Tools provide various utilities for manipulating alignments in the SAM format, including sorting, merging, indexing and generating
alignments in a per-position format.
For more info: http://samtools.sourceforge.net/
TopHat is a fast splice junction mapper for RNA-Seq reads. It aligns RNA-Seq reads to mammalian-sized genomes using the ultra
high-throughput short read aligner Bowtie, and then analyzes the mapping results to identify splice junctions between exons.
For more info: http://tophat.cbcb.umd.edu/
Cufflinks assembles transcripts, estimates their abundances, and tests for differential expression and regulation in RNA-Seq samples.
It accepts aligned RNA-Seq reads and assembles the alignments into a parsimonious set of transcripts. Cufflinks then estimates the
relative abundances of these transcripts based on how many reads support each one, taking into account biases in library preparation
For more info: http://cufflinks.cbcb.umd.edu/
GenomeView is a next-generation stand-alone genome browser and editor initiated in the BSB group at VIB and currently developed at Broad
Institute. It provides interactive visualization of sequences, annotation, multiple alignments, syntenic mappings, short read alignments
and more. Many standard file formats are supported and new functionality can be added using a plugin system.
For more info: http://genomeview.org
QIIME (canonically pronounced ‘Chime’) is a pipeline for performing microbial community analysis that integrates many third party tools
which have become standard in the field. QIIME can run on a laptop, a supercomputer, and systems in between such as multicore desktops.
Linux and Mac OS X are supported natively, and Windows, Linux, and Mac OS X are supported via the QIIME virtual box.
For more info: http://qiime.org/
Velvet is a de novo genomic assembler specially designed for taking in short read sequences, removes errors then produces high quality
unique contigs. It then uses paired-end read and long read information, when available, to retrieve the repeated areas between contigs.
For more info: http://www.ebi.ac.uk/~zerbino/velvet/
Galaxy is a web-based tool that enables researchers to use NGS tools and create workflows
in a web-based environment.
For more info: http://galaxyproject.org
Tufts Researchers can elect to have their sequence data deposited directly into the Tufts Galaxy Server.
DATA ANALYSIS SERVICE PROVIDERS
With GenomeQuest, researchers can:
- Store and interactively analyze all their NGS run results online
- Compare and combine their results with sequence/annotation data from the world's largest collection of sequence reference databases
- Use standard workflows to perform discovery (ChIP-Seq, RNA-Seq (Including Junction Discovery), Whole Genome Analysis, Metagenomics, etc.)
- Configure these built-in workflows or create new workflows
- Share and aggregate their results with lab members and collaborators
- Interface with other popular tools and systems -- including visualization, pathway analysis, LIMs, etc.
- Read from all popular instruments, including Life, Illumina, Roche, and more
- Manage sequence data across all related projects.
Sign up for a Free Basic Account: http://www.genomequest.com
Next-gen sequence analysis and visualization, researchers can:
- On-demand compute and storage infrastructure
- Access everything through your web browser
- Long-term data storage and instant sharing
- Interactive genomic visualization
- RNA-Seq, ChIP-Seq, tag-based analysis
- Sequence variation detection
- Illumina, SOLiD, and Complete Genomics support
Sign up for a Free Basic Account: https://dnanexus.com