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Other TUCF Core Services
Business Hours
Monday to Friday
9:00AM to 5:00PM
(Phone) 617.636.3992
(Fax) 617.636.6737
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Illumina HiSeq 2500 Services
The HiSeq instrument is being shutdown on 11/30/2022
Illumina has obsoleted the HiSeq and will stop selling HiSeq reagents on 11/30/2022 so at that time we will stop taking orders for HiSeq runs. The NovaSeq is the replacement instrument for equivalent or greater amounts of data. Please contact us at tucf-genomics@tufts.edu to discuss considerations for transitioning from the HiSeq to the NovaSeq.
Overview of Instrument
The HiSeq 2500 System features two run modes, rapid run and high output run mode, and the ability to process one or two flow cells simultaneously. This provides a flexible and scalable platform that supports the broadest range of applications and study sizes. No other system lets you perform so many applications so quickly and easily. Choose between rapid run and high output modes offering scalable output to fit your project needs. New reagents enable the generation of up to 1 terabase (Tb) of data in high output mode, supporting the greatest number of samples per run.
Rapid run mode provides quick results.
Rapid run mode provides quick results, allows efficient processing of a limited number of samples, and offers support of longer paired-end 150 base pair reads, which enable greater depth of coverage and improved assembly for de novo applications.
High output mode is perfect for larger studies.
High output mode is ideal for larger studies or when the greatest depth of coverage is required. High output mode allows you to batch process > 6× more samples than rapid mode and to complete large projects quickly and efficiently.
Please click here
for more in-depth information on the HiSeq 2500.
Sequencing Service Charge (Pricing is current and up to date - February 2021)
HiSeq 2500, High Output v4
(8 lanes flow cell), PER LANE |
Tufts |
TMC & External Institutions |
| Single-End 50 (HI-SE50) |
$1,250.00 |
$1,475.00 |
| Single-End 100 (HI-SE100) |
$1,700.00 |
$2,050.00 |
| Single-End 125 (HI-SE125) |
$1,900.00 |
$2,250.00 |
Paired-End 50 (HI-PE50)
(Please call for availability) |
$2,100.00 |
$2,475.00 |
Paired-End 100 (HI-PE100)
(Please call for availability) |
$2,650.00 |
$3,150.00 |
Paired-End 125 (HI-PE125)
(Please call for availability) |
$2,900.00 |
$3,425.00 |
HiSeq 2500, Rapid
(2 lanes) flow cell, PER LANE* |
Tufts |
TMC & External Institutions |
| Single-End 50 (RL-SE50) |
$1,250.00 |
$1,475.00 |
| Single-End 100 (RL-SE100) |
$1,500.00 |
$1,850.00 |
| Single-End 150 (RL-SE150) |
$1,700.00 |
$2,100.00 |
| Paired-End 50 (RL-PE50) |
$1,900.00 |
$2,350.00 |
| Paired-End 100 (RL-PE100) |
$2,400.00 |
$2,900.00 |
| Paired-End 150 (RL-PE150) |
$3,000.00 |
$3,600.00 |
| Paired-End 250 (RL-PE250) |
$4,100.00 |
$4,825.00 |
* Restriction on use of custom sequencing primer.
HiSeq 2500, Rapid
(2 lanes) flow cell, PER FLOW CELL** |
Tufts |
TMC & External Institutions |
| Single-End 50 (RF-SE50) |
$2,150.00 |
$2,525.00 |
| Single-End 100 (RF-SE100) |
$2,600.00 |
$3,125.00 |
| Single-End 150 (RF-SE150) |
$3,075.00 |
$3,600.00 |
| Paired-End 50 (RF-PE50) |
$3,450.00 |
$4,050.00 |
| Paired-End 100 (RF-PE100) |
$4,350.00 |
$5,125.00 |
| Paired-End 150 (RF-PE150) |
$5,750.00 |
$6,750.00 |
** For running a single (multiplexed) samples across the entire flow cell (both lanes). For
order with 2 samples on two indpendent lane, please see the "HiSeq 2500, Rapid (2lanes)
flow cell, PER LANE" section.
Bioinformatics and Data Analysis Services
Please click here for more information.
Data Distribution
FTP Data Folder: RunDate-RunNumber_CustomerName
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